仪器分类
Flow Cytometer_流式细胞仪
Flow Cytometer_流式细胞仪
仪器编号
N/ A
SN序列号
E97501677
生产厂家
BD Biosciences_碧迪
型号
FACS Calibur
制造国家
US_美国
分类号
07060220
放置地点
SA BuildingSA434J-A
购置日期
2010-07-01
入网日期
2020-12-03

主要规格及技术指标

The BD FACSCalibur™ platform allows users to perform both cell analysis and cell sorting in a single benchtop system (cell sorting not included in this machine). The system supports a variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications including enumeration of lymphocyte subsets, stem cells, residual white blood cells and reticulocytes.
Lasers
Air-cooled argon-ion laser, 15 milliwatt, 488 nm; life expectancy >5,000 hours. Optional second laser: nominally 635 nm.
Fluorescence detectors and filters
Up to four high-performance, high dynamic range photomultipliers with bandpass filters: 530 nm (FITC), 585 nm (PE/PI), and >670 nm (PerCP) with base unit, and optional 661 nm (APC) with FL4 option
Dynamic range
Logarithmic amplifiers for SSC, FL1, FL2, FL3, and FL4 (with FL4 option), providing four log decade range.
Fluorescence compensation networks
Compensation for fluorescence spectral overlap between FL1 and FL2, between FL2 and FL3, and between FL3 and FL4 channels (with FL4 option).
Sample concentration
Single-cell suspension of 105 to 2 x 107 particles/mL recommended range.

BD FACSCalibur™平台允许用户在单个台式系统中执行细胞分析和细胞分选(该机器不包括细胞分选)。该系统支持多种研究和临床应用,并辅以一系列直观软件解决方案,可简化各种应用的分析,包括淋巴细胞亚群,干细胞,残留白细胞和网状细胞的计数。
激光
15毫瓦,488 nm氩离子激光器; 预期寿命> 5,000小时。可选的第二激光:标称635 nm。
荧光检测器和滤光片
多达四个带通滤波器的高性能,高动态范围光电倍增器:基本单元为530 nm(FITC),585 nm(PE / PI)和> 670 nm(PerCP),带FL4选件的可选661 nm(APC)
动态范围
用于SSC,FL1,FL2,FL3和FL4的对数放大器(带FL4选件),提供四个对数十进位范围。
荧光补偿网络
补偿FL1和FL2之间,FL2和FL3之间以及FL3和FL4通道之间的荧光光谱重叠(带有FL4选件)。
样品浓度
单细胞悬液建议范围为105至2 x 107颗粒/ mL

主要功能及特色

Wide versatility, multi-parameter measurement, high speed. Widely used in cell biology, oncology, hematology, immunology, pharmacology, genetics and clinical laboratory science, etc.
功能多样,多参数测量,高速。 广泛用于细胞生物学,肿瘤学,血液学,免疫学,药理学,遗传学和临床实验室科学等。

主要附件及配置

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