主要规格及技术指标

The Agilent 6530 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) LC/MS
system with AJS ESI ion source for significantly improved sensitivity, as well as enhanced MassHunter Workstation software for superior data mining and analysis capabilities.
Instrument parameters:
• Sub 1-ppm mass accuracy improves confidence and reduces false positives.
• > 20,000 mass resolution distinguishes target analytes from interferences.
• Up to 5 orders of in-spectrum dynamic range improves detection of trace level targets in the presence of high abundance compounds.
• High femtogram sensitivity enables identification of very low-abundance
compounds.
• Fast data acquisition rates of up to 40 spectra/second ensure maximum
compatibility with fast LC and high throughput methods.
• Wide mass range to 20,000 m/z.

Sample Preparation
1. The concentration of sample is best controlled within 100ppm, 10ppm is recommended to start your analysis.
2. Samples must be clear and transparent, and filtered if necessary.
3. The configuration of the mobile phase should not contain non-volatile substances, such as hydrochloric acid, trifluoroacetic acid, and the salts except ammonium salts. If you are not sure whether your mobile phase can enter the instrument, you can contact the administrator.
4. The mobile phase must be sonicated until no bubbles before use.
5. The mobile phase which contains salt must be filtered with a 0.22μm filter membrane.
LC part
1. When changing the mobile phase, you need to purge first. Binary Pump-pump -purge
2. It is best to bring your own chromatographic column. Otherwise, check the chromatographic column before taking the sample, which is marked whether it is suitable for methanol system or acetonitrile system. If it is another system, please inform the administrator in advance.
3. Check the instrument logbook before testing the sample. If the mobile phase is different, it is best for the instrument to equilibrate with your own mobile phase for more than 10 minutes before injecting the sample.
4. Please switch to the pure organic phase and aqueous phase mode after using the instrument and flush the system and column with a high proportion of organic phase.
Q-TOF part
1. Tuning is required before the first use every day, and the valve of the reference solution needs to be turned off during tuning. The first operation needs to contact the administrator for training.
2. Please do not change the layout of the software.
Agilent 6530精确质量四极杆飞行时间(Q-TOF) LC/MS
该系统采用AJS ESI离子源，显著提高了灵敏度，并增强了MassHunter工作站软件，具有卓越的数据挖掘和分析能力。
仪器参数:
•低于1 ppm的质量精度提高了信心，减少了误报。
•> 20,000质量分辨率区分目标分析物与干扰。
•高达5个数量级的谱内动态范围提高了在高丰度化合物存在时痕量水平目标的检测。
•高飞图灵敏度可以识别极低丰度
化合物。
•快速数据采集率高达40光谱/秒确保最大
兼容快速LC和高通量方法。
•质量范围宽至20,000 m/z。

样品制备
1. 样品浓度最好控制在100ppm以内，建议从10ppm开始分析。
2. 样品必须清澈透明，必要时进行过滤。
3. 流动相的配置不应含有非挥发性物质，如盐酸、三氟乙酸和除铵盐以外的盐。如果您不确定您的移动相位是否可以进入仪器，您可以联系管理员。
4. 流动相在使用前必须经过声波处理，直到没有气泡。
5. 含有盐的流动相必须用0.22μm的过滤膜过滤。
液相部分
1. 当改变流动相时，需要先净化。二元泵-泵-吹扫
2. 最好带上自己的色谱柱。否则，在取样前检查色谱柱，并标明是适用于甲醇体系还是乙腈体系。如果是其他系统，请提前通知管理员。
3. 在测试样品前检查仪器日志。如果流动相不同，最好在进样前用仪器与自己的流动相平衡10分钟以上。
4. 使用仪器后，请切换到纯有机相和水相模式，并用高有机相比例冲洗系统和色谱柱。
Q-TOF部分
1. 每天第一次使用前需要调协，调协时需要关闭参考溶液的阀门。第一次操作需要联系管理员进行培训。
2. 请不要改变软件的布局。

主要功能及特色

The Agilent 6530 Accurate-Mass Q-TOF LC/MS system delivers exceptional MS and MS/MS analyses for applications that demand the highest accurate-mass measurements, without compromising sensitivity and speed. Integration of Agilent Jet Stream Thermal Focusing technology ensures the highest level of sensitivity for your most challenging samples. Powerful new data mining tools facilitate profi ling, characteriza-tion, identifi cation and quantifi cation of compounds in complex mixtures. These performance characteristics enable the 6530 Q-TOF system to support demand-ing applications such as metabolomics, impurity testing, product degradation studies, food safety, and environmental analyses.

主要附件及配置

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