主要规格及技术指标

Technical Specification:
480 instrument Ⅱ；LightCycler 480 Block Cycler Silver Unit,96 well；Software；Sealing Foil Applicator ；LightCycler ⓡ480 Control Unit ；LightCycler 480 Block Kit 384 Silver384 ；Power Cord 1.5m ；

Main configuration:
1.quantitative analysis (absolute quantitative, relative quantitative), 2.meltdown curve analysis, 3.end-point analysis, 4.negative and positive interpretation；
Sample volume : 96-well thermal block cycler: 10 — 100 µl; 384-well thermal block cycler: 5 — 20 µl Detector： Monochrome CCD camera; Resolution 1024 × 1344 pixel; Integration time 10 ms to 10 s Integration time selection Dynamic or manual; Sensitivity < 0.2 nmol/l fluorescein, typically 0.1 nmol/l (20 µl reaction volume) Reproducibility CV ≤ 0.15% Crosstalk well-to-well < 0.2% optically < 0.02% with software correction Temperature control Peltier-based heating and cooling ; Temperature range +37 to +99°C +20°C starting temperature to perform specific Melting Curve analysis if required ; Heating rate : 96-well block: 4.4°C/s; 384-well block: 4.8°C/s ; Cooling rate 96-well block: 2.2°C/s ; 384-well block: 2.5°C/s.

Main features:
Precision: Distinguishes 1.5-fold concentration differences with a confidence level ≥99.8%.
Sensitivity: Capable of detecting single-copy genes.
Dynamic Range: Simultaneously detects 1 to 10¹⁰ copies of DNA.
High Reproducibility: CV < 0.15%.
Analysis Speed: Completes 40 PCR cycles in 40 minutes (384-well plate).
Therma-Base™ Thermal Cycling Technology: Utilizes a silver thermal cycling module with excellent thermal conductivity and an advanced optical detection system to completely eliminate edge effects, ensuring stable and high-quality results. Throughout the process, no internal reference dyes such as ROX are required, effectively reducing costs.
Versatile Analysis Modes: Supports various analysis modes, including absolute quantification, relative quantification, genotyping (melting curve and endpoint methods), and high-resolution melting curve analysis. Compatible with mainstream detection methods on the market, such as dye-based detection, hydrolysis probes (TaqMan® probes), hybridization probes, and simple probes.
Open Platform: Compatible with most common fluorescent dyes on the market, as well as third-party 8-tube strips, 96-well plates, and 384-well plates.
Flexibility: Interchangeable 96-well and 384-well heating modules that users can replace themselves without requiring an engineer's assistance.

主要规格：
主机；96孔模板；软件；亚膜板；控制单元；384孔模板；电源线；

主要技术指标：
1.常规定性定量（绝对定量、相对定量）；2.熔解曲线分析；3.终点法基因分析；4.阴阳性判读功能 ；
样品体积: 96孔热块循环仪:10 — 100 µl; 384孔热块循环仪：5 — 20 µl
探测器: 单色CCD相机，分辨率1024 × 1344 像素，积分时间10 ms至10 s
积分时间选择：动态或手动，灵敏度 < 0.2 nmol/l 荧光素，通常为0.1 nmol/l（20 µl反应体积）; 重现性 CV ≤ 0.15%; 孔间串扰 < 0.2%（光学）< 0.02%（软件校正后）温度控制：基于佩尔帖的加热和冷却; 温度范围：+37 至 +99°C
+20°C起始温度以执行特定的熔解曲线分析（如需要）;加热速率：96孔块：4.4°C/s; 384孔块：4.8°C/s; 冷却速率：96孔块：2.2°C/s; 384孔块：2.5°C/s.

主要特点：
精密度 : 区分1.5倍浓度差异，置信度≥99.8% 灵敏度 : 可检测单拷贝基因 动力学范围: 可同时检测到1-1010个拷贝DNA 高重复性 : CV＜0.15% 分析速度: 40分钟完成40个PCR循环(384孔板) 。采用Therma-BaseTM热循环技术、导热性能好的银质热循环模块和先进的光学检测系 统，彻底消除边缘效应，保持稳定优质的检测结果，且整个过程中无需使用ROX等内参染料，有效节约成本 全能 分析模式多样，绝对定量、相对定量、基因分型(熔解曲线法及终点法)、高分辨率熔解曲线分析，同时适用市面上 主流的检测模式(染料检测、水解探针(TaqMan®探针)、杂交探针、简单探针等) 开放式平台 可使用市面上绝大多数常见荧光染料以及第三方提供的8联管、96孔板和384孔板 灵活 可互换的96孔、384孔加热模块，用户可自行更换，无需工程师到场 .

主要功能及特色

The LightCycler® 480 Instrument II is a rapid, plate-based, thermal block cycler with integrated real-time, online detection capabilities. Based on a comprehensive improvement of Peltier-based technology incorporated in a silver block, the instrument provides extraordinary well-to-well temperature homogeneity and inter-well, inter-cycle reproducibility. The special arrangement of optical components in the LightCycler® 480 Instrument II ensures uniform collection of signals across the plate and makes analysis independent of the sample position on the plate.
The LightCycler® 480 System setup enables the use of all current probe formats (e.g., SYBR Green I, ResoLight dye for high-resolution melting, hydrolysis probes, HybProbe probes, SimpleProbe probes), providing a solution for fast and precise qualitative or quantitative detection of nucleic acids, genotyping, and mutation scanning. Channels and filters can be combined in different ways to allow flexibility in mono-color as well as multiplex assays.

LightCycler® 480 Instrument II 是一款快速的平板式热块循环仪，具有集成的实时在线检测功能。该仪器基于对银块中佩尔帖技术的全面改进，提供了卓越的孔间温度均匀性和孔间、循环间的重现性。LightCycler® 480 Instrument II 中光学组件的特殊排列确保了信号在整个平板上的均匀收集，使分析不受样品在平板上位置的影响。
LightCycler® 480 系统的设置支持所有当前探针格式（例如，SYBR Green I、高分辨率熔解的 ResoLight 染料、水解探针、HybProbe 探针、SimpleProbe 探针），为核酸的快速、精确定性或定量检测、基因分型和突变扫描提供了解决方案。通道和滤光片可以以不同方式组合，以允许在单色和多重检测中灵活使用。

主要附件及配置

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