主要规格及技术指标

LC model: Agilent 1260 prime
Pump: Quaternary Pump
Limit pressure: 800 bar
Flow rate: 0.1-0.5 ml/min
Injection volume: 0.1-100 μl
Detection:
1. UV (190-425nm VWD)
2. TQ: The Agilent Ultivo triple quadrupole mass spectrometer system is equipped with Jet Stream Technology Ion Source and linked to Agilent 1260 prime HPLC.
Instrument parameters:
1. Quadrupole Resolution (FWHM)
2. 0.7 Da Unit mass, 1.2 Da Width = 1.2 Da, Maximum width = 2.5 Da
3. Scanning speed,15000 daltons/sec.
4. Mass detection range: m/z 5-1400.
5. Testing concentration 1-10ppm.

Sample preparation:
1. The concentration of sample is best controlled within 10 ppm, 2 ppm is recommended to start your analysis, and 1 ppm is enough for standards.
2. LCMS grade solvents must be used for the sample preparation.
3. Samples must be clear and transparent, and filtered if necessary.
4. Sample can only dissolve in ACN, MeOH, H2O. If your sample is insoluble in these solvents, you can dissolve it in other solution as little as possible, then dilute it in the suggested solution. In this case, you must inform the Technician which solution you used.

Mobile phase preparation:
The configuration of the mobile phase should not contain non-volatile substances, such as hydrochloric acid, trifluoroacetic acid, and the salts except ammonium salts. If you are not sure whether your mobile phase can enter the instrument, you can contact the administrator.
1. The mobile phase must be ultra-sonicated until no bubbles before use.
2. The mobile phase which contains salt must be filtered with a 0.22μm filter membrane.
3. The mobile phase in P202 is usually ACN, H2O and MeOH, if you want to use other mobile phase, please contact administrator.

LC Columns:
Users should take their own column for their project, MS Lab can only provide C18 50mm 1.8μm for separation of small molecules and short peptides.

液相型号: Agilent 1260 prime
泵：四元泵
最高压：800 bar
流速范围: 0.1-0.5 ml/min
进样量: 0.1-100 μl
检测器:
1. 紫外 (190-425nm VWD)
2. 三重四极杆质谱，配备安捷伦1260色谱仪进行样品分离。
仪器参数：
1. 四极分辨率(FWHM)
2. 0.7 Da单位质量，1.2 Da宽度= 1.2 Da，最大宽度= 2.5 Da
3. 扫描速度，15000道尔顿/秒。
4. 质量检测范围:m/z 5-1400。
5. 测试浓度1-10ppm。
样品制备:
1. 样品浓度建议控制在 10 ppm 以内，开始分析时建议浓度为 2 ppm，标准品浓度为 1 ppm 即可。
2. 样品制备必须使用 LC-MS 级别溶剂。
3. 样品必须清澈透明，必要时进行过滤。
4. 样品一般溶于 ACN、MeOH 和 H2O。如果您的样品不溶于这些溶剂，您可以尽可能少地将其溶解于其他溶液中，然后建议用常用溶液稀释。在这种情况下，您必须告知技术员您使用的是哪种溶液。
流动相制备：
1. 流动相的配置不应含有非挥发性物质，如盐酸、三氟乙酸和除铵盐以外的盐类。如果您不确定您的流动相是否可以进入仪器，可以联系管理员。
2. 使用前必须对流动相进行超声处理直至无气泡。
3. 含盐的流动相必须用 0.22μm 滤膜过滤。
4. P202 中的流动相通常为 ACN、H2O 和 MeOH，如需使用其他流动相，请联系管理员。
色谱柱：
用户应根据自己的项目自备色谱柱，实验室仅提供 C18 50mm 1.8μm 柱子用于小分子物质的分离。

主要功能及特色

The Agilent Ultivo triple quadrupole mass spectrometer system is equipped with Jet Stream Technology Ion Source and linked to Agilent 1260 prime HPLC. The system was designed for profiling, identifying, and characterizing low molecular-weight compounds. And the MassHunter software is applied in Agilent LC-TQ system.
High sensitivity and selectivity.
Ability to perform multiple reaction monitoring (MRM).
Ability to analyze complex mixtures.
High resolution.
Applications: Quantitative analysis.
灵敏度和选择性高。
具备多反应监测(MRM)能力。
能够分析复杂的混合物。
高分辨率。
用途:小分子代谢物的定量分析。

主要附件及配置

无