主要规格及技术指标

The CLARIOstar Plus is a multifunctional microplate reader.
Detection Modes: Fluorescence Intensity –including FRET; Luminescence (flash and glow) – including BRET; UV/Vis Absorbance; Fluorescence Polarization /Anisotropy; Time-Resolved Fluorescence –including TR-FRET; AlphaScreen® / AlphaLISA® /AlphaPlexTM;
Measurement Modes: Top and Bottom reading; Endpoint and Kinetic Measurements; Spectral Scanning (Fluorescence, Luminescence and Absorbance);Sequential multi-excitation; Sequential multi-emission; Ratiometric measurements; Well Scanning.
Microplate Format: 6- to 1536-well plates; LVis Plate with 16 low volume; microspots (2 μL).
Light Sources: High-energy xenon flash lamp; Dedicated Laser for AlphaScreen®/ AlphaLISA® /AlphaPlexTM.
Optical Filters: Top and Bottom for all detection modes; except absorbance; Up to 4 excitation filters, 4 emission filters; 3 dichroic mirrors; Spectral Range: 220 - 1000 nm.
Reagent Injection: Up to 2 built-in reagent injectors. Individual injection volumes for each well: 3 to 500 µL .Variable injection speed up to 420 µL/s.
CLARIOstar Plus是一款多功能微孔板读取器。
检测模式：荧光强度-包括FRET ；发光(闪光和发光)-包括BRET ；紫外/可见吸光度 ；荧光偏振/各向异性 ；时间分辨荧光-包括TR-FRET ； AlphaScreen®/ AlphaLISA®/AlphaPlexTM
测量模式：顶部和底部读数；-终点和动力学测量；光谱扫描(荧光、发光及吸光度)；连续多激发光检测；连续多发射光检测；比率测量；孔扫描。

微孔板类型：6-6536孔板，16个小体积LVis板，微点（2μL）

光源：高强度闪烁氙灯； 适用于AlphaScreen®/AlphaLISA®/ AlphaPlexTM的专用激光器。
光学滤光片:所有检测模式的顶部和底部检测，;除了吸光度;多达4个激励滤波器，4个发射滤波器;3个二向色镜;光谱范围:220 - 1000纳米。
试剂进样：两个内置试剂注射器。每个的单独注射量: 3-500µL。可变注射速度高达420µL/s。

主要功能及特色

CLARIOstar Plus caters for applications ranging from cell biology, signal transduction, immunology to kinetics research:
(1) Functional cell receptor ligand binding analysis: such as GPCR receptor analysis, cytotoxicity, cell survival rate, cell number measurement, calcium absorption, reporter gene, bacterophagy, etc.;
(2) Kinetic analysis: protein aggregation analysis in neurodegenerative diseases, mechanism study of ischemia reperfusion injury in cerebral ischemia diseases, enzyme activity, measurement of Ca2+ and other ion channels;
(3) Kinase and signal transduction pathway detection;
(4) Molecular interaction detection: double ratio detection, such as BRET(bioluminescent energy resonance transfer);
(5) Molecular biology: hybridization, DNA quantification, gene expression, PCR product quantification, SNP analysis, etc

CLARIOstar Plus满足从细胞生物学，信号转导，免疫学到动力学研究的各种应用：
（1）功能细胞受体配体结合分析：如GPCR受体分析，细胞毒性，细胞存活率，细胞数量测定，钙吸收，报告基因，噬菌作用等；
（2）动力学分析：神经退行性疾病蛋白聚集分析，脑缺血疾病缺血再灌注损伤机制研究，酶活性，Ca2+和其他离子通道的测量；
（3）激酶及信号转导通路检测；
（4）分子相互作用检测：双比率检测，如BRET(生物发光能量共振转移) ；
（5）分子生物学：杂交，DNA定量，基因表达，PCR产物定量，SNP分析等

主要附件及配置

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