主要规格及技术指标

UHPLC model: Vanquish™ Neo
Pump: Binary Pump
Limit pressure: 1500 bar
Flow rate: 100 nL/min-100μL/min
Injection volume: 0.01μL-25μL
Column Compartment Temperature Range: 5 - 80 °C
Detection:
1. UV (190-425nm VWD)
2. Q-Exactive Plus: This system is a quadrupole-Orbitrap hybrid high-resolution mass spectrometer, integrating high-resolution accurate mass measurement with targeted quantitative capabilities.

Q-Exactive Plus Mass Analyzer: Orbitrap & Quadrupole
Q-Exactive Plus Resolution:
Maximum Resolution (@m/z 200): 140,000 FWHM
High-Speed Resolution (@m/z 200): 17,500 FWHM
Q-Exactive Plus: Mass Accuracy: Internal Standard < 1 ppm; External Standard < 3 ppm
Q-Exactive Plus: Mass Scan Range: m/z 50 - 6,000
Q-Exactive Plus: Scanning Speed: Up to 12 Hz (at 17,500 FWHM)
Q-Exactive Plus: Quadrupole Mass Filter: Mass Selection Range m/z 50 - 2,000, Isolation Width 0.4 - 10 Da
Q-Exactive Plus: HCD Collision Cell: Collision Energy Range 10 - 180 eV


液相色谱系统：Vanquish™ Neo
泵：二元泵
最高压：1500 bar
流速范围: 100 nL/min-100μL/min
进样量: 0.01μL-25μL
温控范围：5 - 80 °C
检测器:
1. 紫外 (190-425nm VWD)
2. Q-Exactive Plus：本系统为四极杆-轨道阱杂交型高分辨质谱仪，集高分辨精准质量测定与目标化合物定量能力于一体。
Q-Exactive Plus质量分析器： 轨道阱 + 四极杆
Q-Exactive Plus分辨率：
最高分辨率 (@m/z 200)： 140,000 FWHM
高速分辨率 (@m/z 200)： 17,500 FWHM
Q-Exactive Plus质量精度： 内标法 < 1 ppm； 外标法 < 3 ppm
Q-Exactive Plus质量扫描范围： m/z 50 - 6,000
Q-Exactive Plus扫描速率： 最高 12 Hz (在17,500 FWHM下)
Q-Exactive Plus四极杆质量过滤器： 质量筛选范围 m/z 50 - 2,000， 隔离窗宽 0.4 - 10 Da
Q-Exactive Plus 的HCD碰撞池： 能量范围 10 - 180 eV

主要功能及特色

The Q Exactive Plus system is applicable across a range of protein analysis domains, from antibody characterisation to cell- or tissue-based proteomics, to targeted protein quantification or DIA-based protein quantification. Beyond delivering enhanced sensitivity and precision in protein identification applications, it also achieves the high sensitivity required for targeted protein quantification. Its high-resolution accurate mass data offers exceptional confidence and can be utilised for isotope resolution. In small molecule metabolite research applications, it enables non-targeted high-throughput, high-confidence qualitative identification and high-throughput quantitative analysis of small molecule metabolites. By investigating changes in the types and quantities of small molecule metabolites, it assists in understanding their variations and impacts within key life science processes, as well as in lipidomics research and other fields.

Q Exactive Plus 系统可应用于一系列蛋白质分析领域，从抗体表征到基于细胞或组织的蛋白质组学，到目标蛋白质定量或基于DIA 的蛋白质定量。除了在蛋白质鉴定应用具有更高的灵敏度和精确度，同时还能够实现目标蛋白质定量分析所需的高灵敏度，其高分辨准确质量数据具有极高的的置信度，还可用于进行同位素解析。 在小分子代谢物的研究应用中，能够实现非靶向高通量高可信度定性鉴定、小分子代谢物的高通量定量，通过研究小分子代谢物种类以及量的变化去协助了解生命科学关键过程中代谢物在其中的变化及其影响，脂质组学研究等等。

主要附件及配置

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