1. Tank, core, and clamps
Tank: molded polycarbonate;
core: molded polysulfone;
clamps: molded glass-filled polycarbonate
2. Gradient former
Cast acrylic and aluminum
3. Lid:Polycarbonate
4. Electrodes:0.010" diameter platinum
5. Electrical leads:Flexible, straight
6. Glass plates:20 x 20 cm (20 cm format inner plate)
7. Gel sizes:16 x 20 cm (maximum two per run)
8. Spacers available:0.75, 1.0, 1.5 mm
9. Combs 16, 20, 25, and 32 (1 mm only) well combs, and 1-well comb (prep comb for perpendicular gradient gels)
10. Casting stand
Casts two 16 x 16 cm, two 16 x 10 cm, two 16 x 20 cm, or four 7.5 x 10 cm gels per setup
11. Heater and control:Temperature control (PID type) ±0.5°C variation within gel area, ±0.5°C actual in the range of 45–70°C
12. Maximum set temperature:70.5°C
13. DC voltage/power limit:500 V DC voltage limit; 50 W power limit.
1. 电泳槽、核心部件和管夹
电泳槽: 模压聚碳酸酯;
模芯: 模压聚砜;
管夹: 填充了玻璃的膜压聚碳酸酯
2.梯度仪:灌制丙烯酸和铝
3.盖子:聚碳酸酯
4.电极:直径为 0.010" 的铂金丝
5.电导线:灵活,直接
6.玻璃板:20 x 20 cm(20 cm 格式内板)
7.凝胶尺寸:16 x 20 cm(每次最多两个)
8.可用的间隔条:0.75、1.0、1.5 mm
9.梳:16、20、25 和 32 (仅限 1 mm)孔梳和 1 孔梳(制备梳适用于垂直梯度胶)
10.灌胶架:根据设置灌制两个 16 x 16 cm、两个 16 x 10 cm、两个 16 x 20 cm 或四个 7.5 x 10 cm 凝胶
11.加热器和控制:凝胶区域中的温度控制(PID 类型)±0.5°C 变化,45–70°C 范围内实际上下波动 0.5°C
12.最高设置温度:70.5°C
13.直流电压/功率限制:500 V 直流电压限值;50 W 功率限值
The search for unknown mutations in genomic DNA is important for studying the genetic basis of diseases and disorders, including cancer. Additionally, examining DNA polymorphisms is useful for ecological and evolutionary studies of terrestrial, marine, and microbial organisms, with applications ranging from species identification to delineation of population structure to monitoring genetic diversity.
The DCode universal mutation detection system can scan for single-base changes by any of the following electrophoretic techniques:
Single-stranded conformation polymorphism (SSCP)
Denaturing gradient gel electrophoresis (DGGE)
Constant denaturing gel electrophoresis (CDGE)
Temporal temperature gradient gel electrophoresis (TTGE)
Flexible and powerful, the DCode system is the one electrophoresis system that can perform any combination of these techniques. At the center of the system is the temperature control module (which includes a microprocessor-controlled heater, a buffer-recirculating pump, and a stirrer). For techniques requiring accurate temperature control, such as SSCP and TTGE, the gels are immersed in the buffer, and temperatures are regulated between 5° and 70°C. Any run temperature below ambient can be achieved with the cooling tank used in conjunction with an external laboratory chiller. The DCode system can run 64 samples in as little as 2 hours — a major consideration when you're screening DNA for sequence variations.
You can configure your DCode system to grow as your needs grow. Each system includes a vertical electrophoresis cell and choice of adaptor kits for SSCP, DGGE, and CDGE, as well as for TTGE, a technique codeveloped by Bio-Rad. TTGE has all the benefits of DGGE and CDGE without chemical denaturant gradients.
This flexibility lets you quickly add new research tools without investing in new instrumentation. Systems configured for the primary application can be expanded to perform any combination of methods by supplementing the core system with technique-specific adaptor kits listed on the accessories tab. Product options include the complete DCode system and individual DCode systems for DDGE, CDGE, TTGE, and SSCP.
筛选染色体 DNA 中的未知突变对于研究疾病、紊乱(包括癌症)的遗传基础非常重要。 此外,检查 DNA 多态性对陆地生物、海洋生物和微生物的生态和进化研究大有帮助,应用范围包括鉴定物种、种群结构和监控遗传多样性。
DCode 通用突变检测系统可采用以下任一电泳技术检测单碱基变化:
单链构象多态性 (SSCP)
变性梯度凝胶电泳 (DGGE)
均一变性凝胶电泳 (CDGE)
时间温度梯度电泳 (TTGE)
DCode 系统灵活且功能强大,是可以执行任何技术组合的电泳系统。 系统中央是温度控制模块(该模块包括一个由微处理器控制的加热器、一个缓冲液再循环泵和一个搅拌器)。 对于需要精确温度控制的技术(例如 SSCP 和 TTGE),请将凝胶浸入缓冲液中,并将温度调节在 5° 和 70°C 之间。将冷却槽与外部实验室冷却器结合使用可以实现低于环境温度的任何运行温度。 DCode 系统运行 64 份样品只需 2 小时 — 筛选 DNA 以了解序列变化时,可重点考虑该系统。
您可以根据实验需求对DCode 系统进行相应配置。 每个系统包括一个垂直电泳槽和各种针对 SSCP、DGGE、CDGE 和 TTGE(一种由 Bio-Rad 共同研发的技术)所适用的试剂盒。 作为一种由 Bio-Rad 共同研发的技术,TTGE 具有 DGGE 和 CDGE 的所有优点且不需要化学变性剂梯度。
这种系统的灵活性使您能够快速添加新的研究工具而不需要投资购买新仪器。 通过使用“Accessories”(配件)选项卡上列出的特定技术的适配试剂盒补充核心系统,可将检测系统扩展为执行任意检测方法的系统组合。 产品选项包括完整的 DCode 系统及针对不同电泳技术(DDGE、CDGE、TTGE 和 SSCP)的 DCode 系统。
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