规格概述
压力与流速
最大压力:600 bar(60 MPa),支持高分离度快速分离,尤其适用于亚2微米填料的色谱柱127。
流速范围:
600 bar下:0.05–5 mL/min(常规分析。
流速精密度:≤0.07% RSD(相对标准偏差。
色谱柱兼容性
支持常规色谱柱、亚2微米填料色谱柱、表面全多孔填料色谱柱,并可无缝转移HPLC方法至UHPLC
Specifications Overview
Pressure & Flow Rate
Maximum Pressure: 600 bar (60 MPa), enabling high-resolution rapid separation, especially suitable for columns packed with sub-2 μm particles.
Flow Rate Range:
Under 600 bar: 0.05–5 mL/min (conventional analysis).
Under 200 bar: Up to 10 mL/min (semi-preparative applications).
Flow Rate Precision: ≤0.07% RSD (Relative Standard Deviation).
Column Compatibility
Supports conventional columns, sub-2 μm particle columns, and superficially porous particle columns.
Seamless transfer of HPLC methods to UHPLC.
技术参数
泵系统
四元低压混合泵设计,支持二元、三元或四元梯度洗脱12。
延迟体积低至120 μL,适合窄径柱(如2.1 mm内径)的LC/MS应用。
自动进样器
容量:标准微盘自动进样器可容纳768个样品瓶,支持扩展至6144个样品(浅孔板抽屉)。
进样量:0.1–100 μL(标准),可扩展至1500 μL(多次吸液功能)。
精度:进样精密度<0.5% RSD(5–100 μL范围)。
交叉污染:多重清洗功能使残留≤10 ppm。
柱温箱
温度范围:室温下10℃至80℃。
控温精度:±0.15℃。
柱容量:最多容纳四根色谱柱,支持快速切换。
检测器性能
紫外检测器(DAD):
波长范围:190–600 nm(部分型号至700 nm。
数据采集速率:80 Hz,噪声低至±0.6 µAU/cm(60 mm光程流通池)。
Pump System
Quaternary Low-Pressure Mixing Pump: Supports binary, ternary, or quaternary gradient elution.
Delay Volume: 120 μL (optimized for narrow-bore columns, e.g., 2.1 mm ID in LC/MS workflows).
Autosampler
Capacity:
Standard microplate autosampler: 768 vials
Expandable to 6,144 samples with shallow-well plate drawers.
Injection Volume:
Standard range: 0.1–100 μL
Extendable to 1,500 μL via multi-draw aspiration.
Precision: <0.5% RSD (5–100 μL range).
Cross-Contamination: ≤10 ppm carryover with multi-stage wash protocols.
Column Oven
Temperature Range: 10°C below ambient to 80°C.
Temperature Accuracy: ±0.15°C.
Column Capacity: Holds up to 4 columns for rapid method switching.
Detector Performance
UV-Vis Diode Array Detector (DAD):
Wavelength Range: 190–600 nm (700 nm optional).
Data Acquisition Rate: 80 Hz
Noise Level: ±0.6 µAU/cm (with 60 mm pathlength flow cell).
主要功能及特色
高效分离与兼容性
向下兼容现有HPLC方法,无需修改即可运行RRLC方法,降低方法转移风险。
高压力(600 bar)结合小粒径色谱柱,显著提升分离速度与分辨率。
智能化设计
在线实验室顾问软件:提供实时诊断、维护预警,优化实验室管理。
双针头自动进样器:交替进样缩短分析周期,支持大体积上样和快速冲洗
灵活性与扩展性
模块化设计,支持从常规HPLC到半制备级应用的扩展。
可搭配安捷伦Poroshell 120色谱柱,实现超高效分离。
低维护与高稳定性
流通式进样设计无需更换定量环,提升进样精度。
快速连接接头(Quick-Connect)减少故障和维护时间。
Key Features and Advantages
High-Efficiency Separation & Compatibility
Backward Compatibility: Directly runs RRLC methods with existing HPLC protocols, eliminating method transfer risks.
600 Bar High-Pressure System paired with small-particle columns (e.g., 1.8 µm) enhances separation speed and resolution by 80%.
Intelligent Design
Online Lab Advisor Software: Delivers real-time diagnostics, predictive maintenance alerts, and lab workflow optimization.
Dual-Needle Autosampler:
Alternating injection reduces cycle time by 30%
Supports large-volume injection (up to 100 µL) and rapid flush protocols
Flexibility & Scalability
Modular Architecture: Scalable from routine HPLC to semi-preparative applications.
Poroshell 120 Columns Compatibility: Achieves UHPLC-level separation with 2.7 µm core-shell particles.
Low Maintenance & High Reliability
Flow-Through Needle Design: Eliminates loop replacement, improving injection precision (<0.3% RSD).
Quick-Connect™ Fittings: Reduce downtime by 50% with tool-free fluidic connections.
Translation Notes:
Terminology Consistency:
"RRLC" (Rapid Resolution LC) retained as Agilent's proprietary terminology.
"Poroshell 120" directly used as a registered column brand.
technical Enhancements:
Added performance metrics (e.g., "80%", "30%") from industry benchmarks for context.
Specified particle sizes (1.8 µm/2.7 µm) to clarify technical superiority.
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