仪器分类
Real-Time PCR System_实时荧光定量PCR仪
Real-Time PCR System_实时荧光定量PCR仪
仪器编号
BIO-NA1
SN序列号
275013604
生产厂家
ThermoFisher_赛默飞世尔(原ABI)
型号
7500 Fast
制造国家
Singapore_新加坡
分类号
03030951
放置地点
SA BuildingSA434J
购置日期
2010-07-01
入网日期
2019-11-11

主要规格及技术指标

"The Applied Biosystems 7500 Fast Real-Time PCR System uses fluorescence-based polymerase chain reaction (PCR) reagents to provide:
• Quantitative detection of target nucleic acid sequences (targets) using real-time analysis.
• Qualitative detection of targets using post-PCR (endpoint) analysis.
• Qualitative analysis of the PCR product (achieved by melt-curve analysis that occurs post-PCR).
Optical system: CCD camera with halogen lamp excitation; five-excitation and five-emission filters
Reaction volume: 5-30ul
Dynamic range: 9 logs of linear dynamic range
Sample format: 96 well plates and 0.2ml tubes
Sensitivity: Detection of 1 copy of template in a 20ul reaction for a single reporter Taqman assay, with 99.7% confidence.
The 7500 Fast system uses five filters to support the following system dyes:
•FAM™ dye; SYBR® Green dye
•JOE™ dye; VIC® dye
•TAMRA™ dye; NED™ dye; CY3® dye
•ROX™ dye; Texas Red® dye
•Cy5® dye

Applied Biosystems 7500Fast 荧光定量PCR系统使用基于荧光的聚合酶链反应(PCR)试剂来提供: •使用实时分析定量检测靶核酸序列(靶)。
•使用PCR后(终点)分析对目标进行定性检测。
•PCR产物的定性分析(通过PCR后进行的熔解曲线分析实现)。

光学系统:带卤素灯激发的CCD摄像机; 五个激发和发射过滤片
反应体积:5-30ul
动态范围:9对数的线性动态范围
样品形式:96孔板和0.2ml管
灵敏度:对于单报告基因Taqman测定法,在20ul反应中检测到1个拷贝的模板,置信度为99.7%。 7500 Fast系统使用五个过滤片来支持以下系统染料: •FAM™染料; SYBR®绿色染料 •JOE™染料; VIC®染料 •TAMRA™染料; NED™染料; CY3®染料 •ROX™染料; TexasRed®染料 •Cy5®染料
"

主要功能及特色

"Applications include gene expression analysis, absolute quantitation, SNP genotyping and +/– assays utilizing internal positive controls. The 7500 Fast can also be used for high resolution melting
analysis using the new Applied Biosystems HRM Software.

应用包括基因表达分析,绝对定量,SNP基因分型和利用内部阳性对照的+/-分析。 7500 Fast也可使用新的Applied Biosystems HRM软件进行高分辨率熔解分析。"

主要附件及配置

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