[NOTICE 1]
Confocal microscopy training will be conducted on a monthly basis, with each session taking place around the middle of each month. Please refer to the attachment for specific training information.
LSM 880 Airyscan Fast Live Cell is a unique confocal that provides high resolution, sensitivity and speed that enables gentle live cell imaging with super resolution. Airyscan delivers super resolution of 140 nm XY, and 4-8 times sensitivity. Temperature, CO2, humidity controlled incubator is optimized for time lapsing live imaging.
Axio Observer Z1 inverted platform with motorized XYZ stage;
Scan module LSM 880 with Airyscan;
LASERS:
405nm, 458/488/514nm, 543nm, 594nm, 633nm;
OBJECTIVES:
EC Plan-Neofluar 10x/0.3 Ph1 M27
EC Plan-Neofluar 20x/0.50 M27,
EC Plan-Neofluar 40x/0.75 M27,
Plan-Apochromat 63x/1.4 Oil DIC M27
Plan-Apochromat 100x/1.4 Oil DIC M27
DETECTORS:
2 multialkali PMT’s; 1 GaAsP (2x sensitivity); 1 Airyscan super resolution detector; 1 Transmitted light detector
OTHER FUNCTIONS:
Incubator XLmulti S1; Definite Focus; FRET Module; Axiocam 506 image aquisition systen;
Zen Black/Blue software with the modules of Z stacks, timelapse, tiling, multi position, co-localization, FRAP, super resolution, deconvoltuion, 3D processing.
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[NOTICE 1]
共聚焦显微镜培训,将以月度进行,每期在每月中旬15日前后进行。具体培训信息可查看附件内容。
LSM 880 Airyscan共聚焦镜,可实现超高分辨率活细胞成像。 Airyscan提供XY 140纳米的超高分辨率和4-8倍的灵敏度。 温度,二氧化碳,湿度控制的培养箱经过优化,可进行长时间的实时成像。
带有电动XYZ平台的Axio Observer Z1倒置平台;
使用Airyscan扫描模块LSM 880;
激光:
405nm,458/488 / 514nm,543nm,594nm,633nm;
物镜:
EC Plan-Neofluar 10x/0.3 Ph1 M27
EC Plan-Neofluar 20x/0.50 M27,
EC Plan-Neofluar 40x/0.75 M27,
Plan-Apochromat 63x/1.4 Oil DIC M27
Plan-Apochromat 100x/1.4 Oil DIC M27
检测器:
2 multialkali PMT’s; 1 GaAsP (2x sensitivity); 1 Airyscan super resolution detector; 1 Transmitted light detector
活细胞培养装置 XLmulti S1; Definite Focus; FRET模块; Axiocam 506图像采集系统
Zen Black / Blue软件具有Z stacks, timelapse, tiling, multi position, co-localization, FRAP, super resolution, deconvoltuion, 3D processing.
Applications
1. Super resolution confocal imaging of fixed or live samples, photoactivation, or photoconversion in live cells or organisms
2. Capturing fast events such as Ca2+ imaging
3. FRAP
4. FRET
应用领域
1.固定或活体样品的超高分辨率共聚焦成像,活细胞或生物体中的光活化或光转化 / 2.生物学成像,例如Ca2 +成像 / 3. FRAP / 4. FRET
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