主要规格及技术指标

[NOTICE 1]
Confocal microscopy training will be conducted on a monthly basis, with each session taking place around the middle of each month. Please refer to the attachment for specific training information.

The Zeiss LSM880 inverted confocal fluorescence microscope is mainly used for long-term bright-field and fluorescence imaging of cultured living cells or tissues. This system is equipped with Zeiss's unique Airyscan detector, achieving simultaneous improvements in resolution and imaging speed. Airyscan is equipped with a 32-array GaAsP-PMT detector, which has no traditional pinholes. The 32 micro-units of the sensor are like 32 pinholes, each sensing the light from the sample. Through algorithms, the signals measured by the 32 micro-units in all directions are re-summed and repositioned to form a correct and clear image. As there are no pinholes, the light signal is no longer blocked by them. Airyscan can receive more light from the sample. Airyscan can receive 1.25 times the amount of light (1.25 AU) equivalent to that of a traditional pinhole. Each of its 32 micro-units has a sensing effect of 0.2AU, thus significantly improving the signal-to-noise ratio of the image. Simultaneously enhance the image resolution and detail brightness. At a wavelength of 488nm, the detection capability of Airyscan is 1.7 times that of traditional confocal microscopes, with an XY resolution of 140nm and a Z-axis resolution of 400nm.

The inverted microscope is suitable for observing living cells in culture dishes and is equipped with a DIC imaging mode, which can image transparent cells. The equipped live cell incubation system can be matched with 35mm and 60mm culture dishes as well as standard slides, enabling long-term continuous imaging of live cells and high-resolution imaging of fixed samples. It is applicable to observing and recording brain tissue sections, fluorescence labeling of living cells, three-dimensional image reconstruction, qualitative, quantitative, timed and locational distribution detection of cell biological substances and ions, etc., and then analyzing the physiological and biochemical change processes occurring in brain tissue, within cells or between cells.
Axio Observer Z1 inverted platform with motorized XYZ stage;
Scan module LSM 880 with Airyscan;

LASERS:
405nm, 458/488/514nm, 543nm, 594nm, 633nm;

OBJECTIVES:
EC Plan-Neofluar 10x/0.3 Ph1 M27
EC Plan-Neofluar 20x/0.50 M27,
EC Plan-Neofluar 40x/0.75 M27,
Plan-Apochromat 63x/1.4 Oil DIC M27
Plan-Apochromat 100x/1.4 Oil DIC M27

DETECTORS:
2 multialkali PMT’s; 1 GaAsP (2x sensitivity); 1 Airyscan super resolution detector; 1 Transmitted light detector

OTHER FUNCTIONS:
Incubator XLmulti S1; Definite Focus; FRET Module; Axiocam 506 image aquisition systen;

Zen Black/Blue software with the modules of Z stacks, timelapse, tiling, multi position, co-localization, FRAP, super resolution, deconvoltuion, 3D processing.

------------------------------------------------------------
[NOTICE 1]
共聚焦显微镜培训，将以月度进行，每期在每月中旬15日前后进行。具体培训信息可查看附件内容。

Zeiss LSM880倒置共聚焦荧光显微镜主要用于对培养活细胞或组织进行长时间的明场及荧光成像。该系统配备了Zeiss独有的Airyscan探测器，实现了分辨率、成像速度的同时提高。Airyscan拥有一组32阵列的GaAsP-PMT探测器，没有传统的针孔，感测器的32个微单元如同32个针孔，各自感测来自样品的光线，经由演算法重新加总与归位32微单元测得的各方位信号，形成正确的清晰影像。由于没有针孔，光信号不再被针孔遮蔽，Airyscan可接受更多来自样品的光线，Airyscan接受相当于传统针孔1.25倍的光线（1.25 AU），其32个微单元每一个相当于0.2AU的感测效果，因此可以大幅改善影像的信噪比，同时提升影像解析度与细节亮度。在波长488nm下，Airyscan的探测能力是传统共聚焦显微镜的1.7倍，XY分辨率可以达到140nm，Z轴分辨率可达到400nm。

倒置显微镜适用于观察培养皿中的活细胞，配有DIC成像模式，可对透明的细胞进行成像。搭载的活细胞孵育系统，可匹配35mm和60mm培养皿以及标准载玻片，可以对活细胞进行长时间连续成像和对固定样品进行高分辨率成像。适用于观察并记录脑组织切片、活细胞的荧光标记、三维图像重构，细胞生物物质、离子的定性、定量、定时和定位分布检测等，进而分析脑组织、细胞内或细胞间发生的生理、生化变化过程。

带有电动XYZ平台的Axio Observer Z1倒置平台；
使用Airyscan扫描模块LSM 880；

激光:
405nm，458/488 / 514nm，543nm，594nm，633nm；

物镜：
EC Plan-Neofluar 10x/0.3 Ph1 M27
EC Plan-Neofluar 20x/0.50 M27,
EC Plan-Neofluar 40x/0.75 M27,
Plan-Apochromat 63x/1.4 Oil DIC M27
Plan-Apochromat 100x/1.4 Oil DIC M27

检测器:
2 multialkali PMT’s; 1 GaAsP (2x sensitivity); 1 Airyscan super resolution detector; 1 Transmitted light detector

活细胞培养装置 XLmulti S1; Definite Focus; FRET模块； Axiocam 506图像采集系统

Zen Black / Blue软件具有Z stacks, timelapse, tiling, multi position, co-localization, FRAP, super resolution, deconvoltuion, 3D processing.

主要功能及特色

Applications:
1. Super resolution confocal imaging of fixed or live samples, photoactivation, or photoconversion in live cells or organisms
2. Capturing fast events such as Ca2+ imaging
3. FRAP
4. FRET

应用领域
1.固定或活体样品的超高分辨率共聚焦成像，活细胞或生物体中的光活化或光转化
2.生物学成像，例如Ca2 +成像
3. FRAP
4. FRET

主要附件及配置

Adaptors:
1. Carbon dioxide incubator
2. 35mm and 60mm heated imaging adapters
3. Slide imaging adapter
4. Multi-hole plate imaging adapter

适配器：
1. 二氧化碳培养箱
2. 可加热式35mm和60mm适配器
3. 载玻片拍摄适配器
4. 多孔板拍摄适配器