主要规格及技术指标

The gentleMACS Octo Dissociator with Heaters is a benchtop instrument for fully automated and standardized tissue dissociation, perfusion or homogenization of up to eight samples. Equipped with eight individual heating units and numerous optimized and ready-to-use gentleMACS Programs. It requires specialized single-use dissociation tubes. These tubes feature a cap with a uniquely designed rotor-stator system and a septum-sealed central aperture. Two main types of tubes are available C Tubes: Optimized for efficient and gentle tissue processing, yielding high recovery rates of viable cells. M Tubes: Designed for processing tissue fragments and cells to isolate subcellular components (e.g., nucleic acids). Both tubes include a septum-sealed central aperture, ensuring sterile, closed-system handling without the need to open the tube.
配有加热器的gentleMACS Octo组织解离器是一款桌面仪器，可用于完全自动化和标准化的组织解离、灌注或均质化，最多可处理八个样本。该仪器配备了八个独立的加热单元以及多种优化和现成可用的gentleMACS程序。。 Gentle MACS Dissociator需要使用专门的一次性分离管。该管的管盖上带有特殊设计的转子及定子，中心有隔膜封闭的小孔。 主要有两种分离管，C管和M管。C管可以有效、温和地处理组织结构，得到高回收率的活细胞。M管可以处理组织块和细胞，用于分离亚细胞组分，例如核酸。这两种管的中心都有一个隔膜封闭的小孔，能保证在无菌条件下，在密闭系统中安全地处理样品。

主要功能及特色

The gentleMACS Dissociator enables convenient, gentle, safe, time-saving, and standardized automated isolation of single-cell suspensions from tissues or homogenization of tissue fragments. Workflow: Load the sample and appropriate solutions (buffer, culture medium, etc.) into the tube. Invert the tube and attach it to the gentleMACS Dissociator. Select a pre-installed gentleMACS program (customizable for time, speed, and motion direction) and run the protocol. The instrument comes with preloaded programs, and additional protocols or software upgrades can be installed via the gentleMACS Program Card


Applications of gentleMACS Systems
1. Single-Cell Suspension Preparation
Using the gentleMACS C Tube to dissociate tissue samples yields high-activity single-cell suspensions with high recovery rates and intact surface antigen epitopes, ensuring compatibility with downstream applications.
Suitable Tissues/Organisms:
• Solid Tumors: Human primary and metastatic tumors, biopsy samples, murine tumors, and patient-derived xenografts (PDX)
• Neural Tissues: Adult and neonatal rodent neural tissues
• Immune/Soft Tissues: Spleen, lymph nodes, lungs, intestines (including lamina propria), and liver
• Cardiac Tissues: Adult and neonatal rodent hearts
• Muscle Tissues: Skeletal muscle
• Kidneys: Human and murine
• Embryoid Bodies: For stem cell studies
• Other Common Tissues: As required
Nuclear Suspension Preparation:
• Nuclear Extraction Buffer: Rapidly isolates intact nuclei (4°C, 7 minutes) from fresh, frozen, or OCT-embedded tissues. Anti-Nucleus Microbeads enable purification of nuclei for snRNA-seq and ATAC-seq.
• FFPE Tissue Dissociation Kit for RNA Profiling: Efficiently generates intact nuclei from formalin-fixed, paraffin-embedded (FFPE) tissues for snRNA-seq.
2. Tissue Homogenate Preparation
The gentleMACS M Tube facilitates homogenization of tissues for molecular or microbiological analyses. It minimizes cross-contamination and safeguards operators when handling infectious samples.
• Nucleic Acid Extraction: DNA/RNA isolation from fresh or frozen tissues.
• Protein Isolation: For proteomic studies.
• Pathogen Quantification: Detects pathogen loads in clinical or research samples.
3. Ex Vivo Perfusion for Primary Cell Isolation
gentleMACS Perfusion Tube: Used for rodent liver perfusion to isolate high-viability hepatocytes. gentleMACS Perfusion Tube 2: Designed for murine heart perfusion to obtain viable cardiomyocytes.

gentle MACS Dissociator能够以温和的、安全的、省时的、标准的自动化方法很便利地从组织中分离单个细胞悬液，及将组织块制备成匀浆。将样品材料和适宜的溶液（缓冲液、培养基、等等）加入试管内，把试管头朝下安装在gentleMACS Dissociator上。从菜单中选择gentleMACS程序，并在仪器上运行。该程序可以进行时间、速度和操作方向的设定。 仪器中安装了一系列操作程序，还可以添加许多实验规程及程序。新开发的程序和升级程序都可以通过gentleMACS程序卡来安装。

适用样品
1. 单细胞悬液制备
通过gentleMACS C管对组织样本进行温和解离，可获得高活性单细胞悬液，具有高回收率和完整的表面抗原表位，适用于后续分析。
适用组织/样本类型：
• 固体肿瘤：人原发性及转移性肿瘤、活检样本、小鼠肿瘤、患者来源类器官（PDX）
• 神经组织：成年及新生期啮齿类动物神经组织
• 免疫/软组织：脾脏、淋巴结、肺脏、肠道（含固有层）、肝脏
• 心脏组织：成年及新生期啮齿类动物心脏
• 肌肉组织：骨骼肌
• 肾脏：人及小鼠样本
• 类胚体：用于干细胞研究
• 其他常规组织：按要求使用
细胞核悬液制备：
• 核提取缓冲液：可在4°C条件下，仅需7分钟快速从新鲜、冷冻或OCT包埋组织中分离完整细胞核。Anti-Nucleus Microbeads可纯化核用于snRNA-seq和ATAC-seq。
• FFPE组织解离试剂盒（用于RNA谱分析）：可高效从福尔马林固定石蜡包埋（FFPE）组织中获取完整细胞核用于snRNA-seq。
2. 组织匀浆制备
gentleMACS M管可实现组织匀浆化，适用于分子或微生物学分析，处理感染性样本时可有效避免交叉污染并保障操作者安全。
• 核酸提取：从新鲜或冷冻组织中分离DNA/RNA
• 蛋白质提取：用于蛋白质组学研究
• 病原体定量：检测临床或研究样本中的病原载量
3. 离体灌注法原代细胞分离
gentleMACS灌注管：用于啮齿类动物肝脏灌注以获取高存活率肝细胞 gentleMACS灌注管2：专为小鼠心脏灌注设计，用于获取高活性心肌细胞

主要附件及配置

Heat UNITS