主要规格及技术指标

XF Pro 系统由台式分析仪和触摸屏控制器组成，由安捷伦Seahorse Wave Pro 软件驱动。该软件支持SeahorseXF检测的所有方面，包括检测设置、仪器控制和数据分析。
1. 硬件系统
检测通道：运行检测需要XF探针板，即与96孔探针板配合使用，该系统测量微孔板的每个孔中的各分析物浓度随时间的变化，并同时自动计算耗氧率(OCR)和质子释放率(PER)(细胞外酸化率(ECAR)。
传感器技术：采用固态、荧光式生物传感器，直接整合在消底部。无需液态试剂，稳定性高，灵敏度高。
测量原理：实时同步检测氧消耗率（OCR） 和细胞外酸化率（ECAR）。
温度控制：精确的37℃恒温控制系统，确保细胞在整个实验过程中处于生理相关环境。
自动液剂添加：每个检测孔配有4个独立的药剂孔，仪器可在实验过程中自动、精确地注入多达4种调节代谢的化合物。
自动化与通量：高度自动化，可自动执行校准、检测和清洗步骤，适合中高通量筛选。
2. 检测性能
检测灵敏度：所需细胞量少，可从数万到数十万个细胞/孔（取决于细胞类型）。
测量速度：可设定快速、重复的混合-测量循环（通常每个循环持续几分钟），提供高时间分辨率的数据点，捕捉代谢的动态变化。
数据质量：提供实时、非侵入性的动力学数据，而非单一的终点读数。
3. 耗材
检测板：细胞培养微孔板。
试剂盒：提供各种经过优化的预配制试剂盒（如 XF Cell Mito Stress Test Kit, XF Glycolysis Stress Test Kit），确保实验的标准化和可重复性。

The XF Pro system, comprising a bench top analyzer and touch screen controller, is driven by Agilent Seahorse Wave Pro software. This software enables all aspects of Seahorse XF assays including assay setup, instrument control, and data analysis.
1. Hardware System
Detection Channels: Operation of the detection requires an XF probe board, which is used in conjunction with a 96-well probe board. This system measures the concentration of each analyte in each well of the microplate over time and simultaneously automatically calculates the oxygen consumption rate (OCR) and proton release rate (PER) (extracellular acidification rate, ECAR).
Sensor Technology: Utilizes solid-state, fluorescent biosensors that are directly integrated into the bottom of the device. No liquid reagents are required, resulting in high stability and sensitivity.
Measurement Principle: Real-time synchronous detection of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
Temperature Control: Precise 37°C constant temperature control system to ensure that cells are in a physiologically relevant environment throughout the experiment.
Automatic Liquid Addition: Each detection well is equipped with 4 independent reagent wells, and the instrument can automatically and precisely inject up to 4 compounds that regulate metabolism during the experiment.
Automation and Throughput: Highly automated, capable of automatically performing calibration, detection, and cleaning steps, suitable for medium to high-throughput screening.
2. Detection Performance
Detection Sensitivity: Minimal number of cells required, ranging from tens of thousands to several hundred thousand cells per well (depending on the cell type).
Measurement Speed: Can set up rapid, repetitive mixing-measurement cycles (typically each cycle lasts for several minutes), providing high time-resolution data points to capture metabolic dynamic changes.
Data Quality: Provides real-time, non-invasive kinetic data rather than a single endpoint reading.
3. Consumables
Detection Plate: Cell culture microplate.
Kit: Provides various optimized pre-prepared kits (such as XF Cell Mitochondria Stress Test Kit, XF Glycolysis Stress Test Kit), ensuring the standardization and reproducibility of the experiment.

主要功能及特色

1.功能：
Seahorse XF Pro 细胞代谢分析仪能够通过实时、无标记地检测活细胞的耗氧率和酸化率，来精准解析线粒体功能和糖酵解功能，应用于细胞代谢研究领域。
1）实时同步检测：OCR (Oxygen Consumption Rate) ：直接反映线粒体呼吸功能（电子传递链活性）。ECAR (Extracellular Acidification Rate) ：主要反映糖酵解通量（乳酸的产生导致酸化）。
2）核心代谢表型分析：通过著名的Stress Test assays，揭示细胞完整的代谢能量图谱：
3）线粒体压力测试：通过顺序注入多种探针药，测量基础呼吸、ATP生产相关呼吸、质子漏、最大呼吸能力、备用呼吸能力等关键参数。
4）糖酵解压力测试：测量糖酵解、糖酵解能力、糖酵解储备等参数。
5）细胞功能评估：评估细胞在生理、病理或药物处理下的代谢适应性和功能健康状况。
2.特色：
1）在细胞保持活性和完整性的前提下，实时监测其代谢变化，提供动态功能数据。
2）无标记、非侵入性：无需对细胞进行基因改造或使用标记物（如荧光染料），避免了这些操作本身对细胞代谢可能产生的干扰
3）操作流程高度标准化：预设计的实验方案和试剂盒使复杂的代谢分析变得简单、可靠且易于重复。
4）应用领域极其广泛：癌症生物学、免疫代谢（如T细胞功能）、神经科学、干细胞生物学、衰老研究等。

1.Function:
The Seahorse XF Pro Cell Metabolism Analyzer can precisely analyze mitochondrial function and glycolytic function by real-time, label-free detection of the oxygen consumption rate and acidification rate of living cells and is applied in the field of cell metabolism research.
1) Real-time synchronous detection: OCR (Oxygen Consumption Rate): directly reflects mitochondrial respiratory function (electron transport chain activity). ECAR (Extracellular Acidification Rate): mainly reflects glycolytic flux (lactic acid production leading to acidification).
2) Core metabolic phenotype analysis: Through well-known Stress Test assays, reveal the complete metabolic energy profile of cells:
3) Mitochondrial stress test: by sequentially injecting multiple probe drugs, measure key parameters such as basal respiration, ATP production-related respiration, proton leakage, maximum respiratory capacity, and reserve respiratory capacity.
4) Glycolytic stress test: measure glycolytic, glycolytic capacity, glycolytic reserve, etc.
5) Cell function assessment: evaluate the metabolic adaptability and functional health status of cells under physiological, pathological or drug treatment.
2. Features:
1) While maintaining the activity and integrity of cells, real-time monitoring of their metabolic changes provides dynamic functional data.
2) Label-free, non-invasive: no need for genetic modification of cells or the use of markers (such as fluorescent dyes), avoiding possible interference to cell metabolism from these operations.
3) Highly standardized operation process: pre-designed experimental schemes and kits make complex metabolic analysis simple, reliable, and easy to repeat.
4) Extremely wide range of application fields: cancer biology, immune metabolism (such as T cell function), neuroscience, stem cell biology, aging research, etc.

主要附件及配置

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