主要规格及技术指标

The BD FACSCelesta™ flow cytometer is designed to make multicolor flow cytometry more accessible and allow researchers to benefit from new innovations in instrument and reagent technology. Parallel advances in optical and reagent technology are pairing multilaser, multidetector instruments with bright new dyes, enabling increasingly deep and powerful insights in cell analysis.The system has been designed to fit on the benchtop and leverages the proven BD FACSDiva™ software to streamline workflow from system setup to data acquisition to data analysis.
3 Lasers: Blue (488nm), Violet (405nm) and Red (640nm)
Laser Power: 405 nm: 50 mW; 488 nm: 20 mW; 640 nm: 40 mW
Forward Scatter Detection: Photodiode detector with a 488/10 bandpass (BP) filter
Side Scatter Detector: Photomultiplier tube (PMT) with a 488/10 BP filter
Emission Optical Design: Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays. The BD FACSCelesta optical pathways use signal reflection to maximize signal detection.
Data Acquisition Rate: 25,000 events/s with beads*
Sample Carryover: <0.3% when acquiring from 12 x 75-mm tubes and running a wash tube between samples

BD FACSCelesta™流式细胞仪旨在使多色流式细胞仪更易于使用，并使研究人员可以从仪器和试剂技术的新创新中受益。 光学和试剂技术的并行进步是将多激光，多检测器仪器与明亮的新型染料配对，从而使细胞分析的见解越来越深入。该系统设计为适合台式，并利用成熟的BD FACSDiva™软件简化了从系统设置到数据采集，数据分析的流程。

3激光：蓝色（488nm），紫色（405nm）和红色（640nm）
激光功率：405 nm：50 mW; 488 nm：20mW; 640 nm：40mW
前向散射检测：带有488/10带通（BP）滤波器的光电二极管检测器
侧面散射检测器：带488/10 BP滤光片的光电倍增管（PMT）
发射光学设计：凝胶耦合比色皿的发射光通过光纤传输到检测器阵列。 BD FACSCelesta光路使用信号反射来最大化信号检测。
数据采集速率：25,000个事件/秒，用磁珠样品
残留：从12 x 75毫米试管中采集并在样品之间运行冲洗管时<0.3％

主要功能及特色

Immunology Applications: See What You've Been Missing
The BD FACSCelesta can rapidly and accurately analyze many kinds of cells, including lymphoid tissue (thymus, spleen, and lymph nodes), digested solid tissues, and blood.Using panels of directly conjugated fluorescent antibodies to recognize specific cell surface and intracellular epitopes, multicolor flow cytometric analysis allows researchers to interrogate specific target protein levels expressed by individual cells in various phases of development and differentiation.
Immunology Applications: Illuminate Rare Cells and Low-Density Antigens
BD Horizon Brilliant™ polymer conjugates can endow previously dim cells with much brighter fluorescence signals than traditional organic fluorescent dyes or even phycobiliproteins such as PE or APC. Optimization for these bright dyes enables the BD FACSCelesta to identify cell populations with a broader range of receptor density than was previously possible.
Cell Biology Applications: Cell Analysis in a New Light
Flow cytometry can provide rich data to cell biologists working in a wide range of fields, from molecular interaction to systems biology, from pharmacokinetics to cancer biology, from cell signaling to marine biology to biophysics. Use of flow cytometry has blossomed with intensified interest in proteomics, increased use of biomarkers in drug development research, and the spread of high-throughput, cell-based drug screening research. With three lasers and up to 14 optical channels, the BD FACSCelesta can multiplex many of these assays at once on the same sample.
Cell Biology Applications: Study Complex Populations at the Single-Cell Level
A major strength of flow cytometry is its ability to study complex populations. Other techniques that examine individual cells, such as microscopy, are hard to scale up for quantitative analysis. Flow cytometry can characterize large numbers of individual cells, allowing it to identify, quantify, and characterize different subsets of cells in a heterogeneous population.
免疫学研究
BD FACSCelesta 能够快速精确的分析各种类型的细胞，包括淋巴组织细胞（胸腺、脾脏以及淋巴结）、消化组织细胞以及血细胞。使用直标的荧光抗体可以检测细胞表面抗原和细胞内抗原，多色流式细胞分析仪使研究者能够检测到细胞在不同生长水平及分化水平所表达的特异性目标蛋白。
照亮罕见的细胞和低密度抗原
BD Horizon Brilliant 可以使本身信号较弱的细胞具有比传统的有机荧光染料或藻胆蛋白（PE、APC）更强的荧光信号。对这些亮度高的染料的优化可以使BD FACSCelesta 用比以往更广泛的受体密度来区分细胞类群。
 细胞生物学研究
流式细胞仪能够提供给各领域工作的细胞生物学家从分子间相互作用到系统生物学，从药物（代谢）动力学到肿瘤生物学，从细胞传导信号到海洋生物学再到生物物理学等多方面的大量数据。流式细胞仪的使用增强了蛋白质组学的研究，提高了药物研发中生物标记物的使用，并发展了高通量的以细胞为基础的药物筛选研究。BDFACSCelesta 配备有三个激光器和高达14个检测器，使您可以针对同一样品对上述多个实验进行同时测定。
 单细胞水平研究复杂的类群
流式细胞仪一个最主要的优势就是它能够用于研究复杂的类群。而其他检测单个细胞的技术，比如显微镜，不能对样品进行定量分析。流式细胞仪能够对大量的单个细胞进行定性定量分析，同时对具有不同特征的细胞亚群也做出特征性鉴定。

主要附件及配置

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