仪器分类
Inverted Fluorescence Microscope_倒置荧光显微镜
Inverted Fluorescence Microscope_倒置荧光显微镜
仪器编号
BIO-NA4
SN序列号
533766
生产厂家
Nikon_尼康
型号
Eclipse Ti-S
制造国家
Japan_日本
分类号
03040108
放置地点
SA BuildingSA434E
购置日期
2010-07-01
入网日期
2020-12-03

主要规格及技术指标

Ti-S fluorescence microscope is an inverted phase contrast fluorescence microscope manufactured by Nikon. It consists of four parts: Ti-S microscope, microscope imaging system (Charge-coupled Device, CCD), bright field light source and fluorescence excitation light source.
1.Optical system: CFI60 infinity optical system
2.Subjective: CFI 10X (22)
3.Objectives: Plan Fluor 4X/0.13; Plan Fluor 10X/0.30; Plan Fluor 20X/0.45; Plan Fluor 40X/0.60
4.Illumination: Halogen lamp (12v 100W)
5.Epi-fluorescence excitation block:
DAPI(EX 340-380; DM 400; BA 435-485);
FITC(EX 465-495; DM 505; BA 515-555);
TRITC(EX 540/25; DM 565; BA 605/55)
6.Mercury Lamp: C-SHG1
Ti-S荧光显微镜是日本尼康公司生产的一台倒置相差荧光显微镜,由Ti-S显微镜、显微镜成像系统(Charge-coupled Device,CCD)、明场光源和荧光激发光源四大部分组成。
1、光学系统:CFI60 无限远光学系统
2、目镜:CFI 10X (22)
3、物镜:4×、10×、20×、40×。
4、照明系统:卤素灯 (12v 100W)
5、落射荧光激发块:
DAPI(EX 340-380; DM 400; BA 435-485);
FITC(EX 465-495; DM 505; BA 515-555);
TRITC(EX 540/25; DM 565; BA 605/55) 
6、光源:汞灯C-SHG1

主要功能及特色

The system can be used for the observation of bright field, dark field, phase contrast, simple polarized light and epi-fluorescence of cell and tissue samples. It can observe and study the fine structure of living cells without affecting the living cell tissue. It can be used for fluorescence detection involving small molecules fluorescence probe and fluorescent protein research, such as subcellular localization, apoptosis detection, immunofluorescence staining, in situ hybridization, high-throughput screening, and photobleaching/photoactivation applications. This microscope is used in conjunction with NIS-Elements imaging software to support various image capture and analysis methods.
该系统可用于细胞和组织样本明场、暗场、相差、简易偏光及落射荧光的观察,可在不影响活细胞组织的情况下观察和研究活细胞的精细结构,可用于涉及小分子荧光探针及荧光蛋白的研究,探针亚细胞定位、细胞凋亡检测、免疫荧光染色、原位杂交、高通量筛选以及光致漂白/光活化等应用。该显微镜与NIS-Elements成像软件结合使用,支持各种图像拍摄、分析方式。

主要附件及配置

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